On FEI Tecnai, Titan and Talos microscopes run with TIA (TEM Imaging and Analysis), each
acquisition is saved as a pair: an .emi file with the microscope settings and one or more
.ser files with the data, named name_1.ser, name_2.ser and so on, one per detector. This
page reads the pair and writes TIFF, PNG and CSV files that any program opens.
How to convert SER and EMI to TIFF
- Upload the
.emifile together with all of its.serfiles, or a.zipof the folder. The files are matched by name. - Choose the picture format, the scale bar position and colour, and how series are handled (average, first frame, or every frame).
- Download a calibrated TIFF and a picture with a scale bar for every detector, plus the settings as text and JSON.
What is converted
- Images from the CCD or the STEM detectors, with the pixel size from the
.serfile. STEM scans with BF, DF and HAADF give one picture per detector. - Image series (previews, tilt or time series) as one multi-page TIFF.
- Diffraction patterns, calibrated in 1/nm and shown on a log scale.
- EDS spectra, line scans and spectrum images: CSV with the energy axis, one column per position for line scans, and for spectrum images the sum spectrum, a total-counts map, element maps for the elements you type and the data cube.
- Settings from the
.emifile: high tension, magnification, camera length, mode, spot size, dwell time, stage position and the full description TIA stores.
Things to watch
- A
.serfile uploaded without its.emiis still converted with its own calibration, but the microscope settings are missing, and a diffraction pattern is then treated as an image. Upload both. - TIA stores images bottom-up; the converter flips them so the pictures look as they did on the TIA screen.
- A spectrum image whose scan was stopped early is converted; the positions that were never scanned stay empty and a note tells you how many hold spectra.
For DigitalMicrograph files from the same microscopes see DM3 and DM4 to TIFF.
Newer Thermo Fisher instruments save .emd: see Velox EMD to TIFF.
More about what this tool reads and writes: Electron Microscopy File Converter (EMD, DM3, DM4, SER, TIFF).